Analysis of Uric Acid Metabolites in Urine (or in synthetic urine) by HPLC-MS

 

Introduction:

This laboratory experiment makes use of HPLC-MS to identify and quantify uric acid, 6-aminouracil (6-AU), and allantoin in synthetic urine, with tyrosine as an internal standard. It is designed for use in Quantitative Analysis laboratory, but may find applications in forensic and biochemistry laboratories as well. An Excel file is provided that serves as a guide to calculations following the experiment.

Analysis (PDF)

Report (Excel)

Developed by:

Dr. Maria Matyska-Pesek

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