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Analysis of Limonin in Orange Juice by HPLC (1)

  Introduction Limonin is a terpenoid compound that is found in various citrus juices. In the citrus fruit itself however, only the limonin precursor limonate-A-ring lactone (LARL) is present. When… Read more »

Analytical Chemistry 2.0: Chromatographic and Electrophoretic Methods

This chapter from David Harvey’s complete e-text covers the general theory of column chromatography, GC, HPLC, and electrophoresis. Available as a downloadable pdf this 102 page chapter includes diagrams, examples… Read more »

(38001) Separation Science

Designed as a faculty resource on separation science, the published materials are available to be used and modified to fit any curricular pedagogy. Materials include a text, sample problems, and… Read more »

(10064) HPLC – free, opensource HPLC simulator project

Want to see what happens in LC, but there’s not an HPLC available for your use? This simulator shows what the important parameters are and lets you see the effect… Read more »

(33001) Intro to Capillary Electrophoresis (lecture slides)

This site contains the complete notes in a PowerPoint-like format for a chemical separations course. This specific selection of notes provides an introduction and overview of capillary electrophoresis and associated… Read more »

(10061) A Microchip Capillary Electrophoresis Experiment for the Instrumental Analysis Laboratory

Microfluidics and electrophoresis are two aspects of bioanalysis that are central to modern instrumentation. This laboratory has students use a microfluidic device for electrophoretic experiments. It takes a microfabrication approach… Read more »

Analytical Chemistry 2.0

A complete textbook for Quantitative Analysis is freely downloadable. Teach yourself Analytical Chemistry, or be part of a class on the subject. The website is available here .

Kinetic Capillary Electrophoresis(KCE): A conceptual Platform for Kinetic Homogeneous Affinity Methods

Kinetic Capillary Electrophoresis is a family of methods for studying binding of biopolymers, measuring rate constants, binding constants, and in some cases providing mechanistic details. This annimation illustrates several kinetic… Read more »

Affinity Chromatography

Using exposition, graphics, and commercial videos, this module teaches the theory and application of affinity chromatography in the characterization of proteins, nucleic acids, and other biochemical/biomedical systems. Problems and application… Read more » Gas Chromatography

This video, distributed on YouTube by the Royal Society of Chemistry is on the basic principles of GC. This video is a short primer (~5 min) which introduces the technique… Read more »

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