A primer for liquid chromatography provided by Agilent Technologies. This publication reviews all aspects of fundamental liquid chromatography (LC). The material covered is similar to might be found in an analytical chemistry textbook. It is especially well-suited for first time users of liquid chromatography. Reviews of fundamental math, instrument design, and method development are included. In…
Designed as a faculty resource on separation science, with a particular emphasis on chromatographic separations. The published materials focus on active learning but may be modified to fit any curricular pedagogy. Materials include a text, sample problems, and an instructor's manual.
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This video, distributed on YouTube by the Royal Society of Chemistry is on the basic principles of GC. This video is a short primer (~5 min) which introduces the technique and instrumentation.
There are many assumptions that are made in the presentation of this material, due to the fact the video is meant to be a…
US Environmental Protection Agency site that explains GC for those interested in environmental analysis. Assumes some user background in the field. Includes a partial list of EPA Field Methods that utilize GC which may be helpful to those interested in regulatory protocols.
The website is available here .
Further Analytical Methods developed by the Environmental…
This site provides a Word document that describes a GC experiment involving the separation and quantitation of four butyl alcohol isomers. It has a complete background about the components of a GC instrument (with labeled pictures) and their functions. It has a section devoted to the measurement of response factors of the detector in…
This site has a series of links to QuickTime movies that animate the chromatographic process. The movies show the effect of changing K, L, and N on peak width and separation. The site is useful in combination with other sources that explain the background theory. The website is available here .
This site contains standard definitions related to chromatgraphy similar to treatments found in analytical textbooks. It introduces the beginning student to LC concepts relevant to biochemistry and includes an example of choosing a mobile phase pH for a protein separation based on ion exchange.
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This site is an introductory lecture in outline form about chromatography. It appears directed toward biochemical separations. The coverage is broad but not deep. Discussions of counter-current separations, gel permeation, and ion exchange are accompanied by some mention of partition, paper, and gas chromatography
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This site contains a good review of GC detectors that is linked to animated files describing the hardware and principles of operation for GC/MS. From this site, links are available to other animations about a variety of analytical topics.
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This chromatography experiment is designed to introduce students to the theory of chromatographic separation of mixtures and packed column chromatography. A section on instrument troubleshooting may be included as an additional optional activity.
The website is available here .