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Author page: ASDL Liquid Chromatography Editor

Analysis of Uric Acid Metabolites in Urine (or in synthetic urine) by HPLC-MS

  Introduction: This laboratory experiment makes use of HPLC-MS to identify and quantify uric acid, 6-aminouracil (6-AU), and allantoin in synthetic urine, with tyrosine as an internal standard. It is designed for use in Quantitative Analysis laboratory, but may find applications in forensic and biochemistry laboratories as well. An Excel file is provided that serves as…

Analytical Chemistry 2.0: Chromatographic and Electrophoretic Methods

This chapter from David Harvey's complete e-text covers the general theory of column chromatography, GC, HPLC, and electrophoresis. Available as a downloadable pdf this 102 page chapter includes diagrams, examples and problems and provides an excellent introduction to separations. The website is available here .

HPLC – free, opensource HPLC simulator project

Want to see what happens in LC, but there's not an HPLC available for your use? This simulator shows what the important parameters are and lets you see the effect of changing those parameters on realistic separations. The website is available here . Note - the software for this application is still functional, but runs on an outdated…

Kinetic Capillary Electrophoresis(KCE): A conceptual Platform for Kinetic Homogeneous Affinity Methods

Kinetic Capillary Electrophoresis is a family of methods for studying binding of biopolymers, measuring rate constants, binding constants, and in some cases providing mechanistic details. This annimation illustrates several kinetic CE modes and their effect on the complex formation and dissociation. The website is available here .


This video, distributed on YouTube by the Royal Society of Chemistry is on the basic principles of HPLC. This video is a short primer (~5 min) which introduces the basic technique and instrumentation. The theory of HPLC is described at a very basic level. Separation theory is presented in a qualitiative fashion, and both the mechanics…

Ion Exchange Chromatography

This website contains an ion chromatography simulator that can be run on-line or can be downloaded. The simulator focuses on separations of proteins using Ion Chromatography. Also included is hyperlinked reference information, an example of a homework assignment using the simulator, and some information on protein structures.(note this is an archived version of this site, as…

Waters Corporation

This is an all-inclusive site in terms of information about HPLC. What may be most useful to students, faculty and early practitioners of HPLC are "New Academia Area" and "Life Science Solutions - New Initiatives." The company application notes are available as pdf files, and the application library is available with a link from the home…
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