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Strategies for Helium Carrier Gas Conservation and Transition to Alternative Carrier Gas

Description: Shannon Coleman, GC Application Scientist from Agilent presents options that address current helium shortages. Strategies for Helium Carrier Gas Conservation and Transition to Alternative Carrier Gas This discussion will focus on understanding tactics for carrier gas conservation, including what hardware and strategies are available for conservation.  Alternative carrier gas options will be explored, as…

Introduction to Mass Spectrometry Video

This video, part of the knowbeetutoring collection available from Wordpress.com offers a basic but effective introduction to ion production of organic molecules in an electron ionization spectrometer. The basis for fragmentation is explained for the propane molecule using very clear graphics. It is an excellent first exposure to the technique, and supplies a very solid…

Analysis of Uric Acid Metabolites in Urine (or in synthetic urine) by HPLC-MS

  Introduction: This laboratory experiment makes use of HPLC-MS to identify and quantify uric acid, 6-aminouracil (6-AU), and allantoin in synthetic urine, with tyrosine as an internal standard. It is designed for use in Quantitative Analysis laboratory, but may find applications in forensic and biochemistry laboratories as well. An Excel file is provided that serves as…

ESTIMATION OF MEASUREMENT UNCERTAINTY IN CHEMICAL ANALYSIS

This site offers an introductory course (as a MOOC) on the estimation of measurement uncertainty specifically related to chemical analysis (analytical chemistry). The course gives the main concepts and mathematical apparatus of measurement uncertainty estimation and introduces two principal approaches to measurement uncertainty estimation – the ISO GUM modeling approach (aa “bottom-up”  approach) and the single-lab…

Raman Spectroscopy Tutorial and FAQs

Horiba scientists wrote this tutorial to help people learn about Raman spectroscopy. The authors write clearly and provide great illustrations to accompany the text. The tutorial addresses the theory of Stokes & anti-Stokes Raman scattering, SERS and other techniques. In addition, the tutorial introduces the instruments needed for Raman experiments - like lasers, optics, and microscopes.…

Separation Science

Designed as a faculty resource on separation science, with a particular emphasis on chromatographic separations.  The published materials focus on active learning but may be modified to fit any curricular pedagogy. Materials include a text, sample problems, and an instructor's manual. The website is available here .

HPLC Simulator.org – free, opensource HPLC simulator project

Want to see what happens in LC, but there's not an HPLC available for your use? This simulator shows what the important parameters are and lets you see the effect of changing those parameters on realistic separations. The website is available here . Note - the software for this application is still functional, but runs on an outdated…
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