A primer for liquid chromatography provided by Agilent Technologies. This publication reviews all aspects of fundamental liquid chromatography (LC). The material covered is similar to might be found in an analytical chemistry textbook. It is especially well-suited for first time users of liquid chromatography. Reviews of fundamental math, instrument design, and method development are included. In…
This video, part of the knowbeetutoring collection available from Wordpress.com offers a basic but effective introduction to ion production of organic molecules in an electron ionization spectrometer. The basis for fragmentation is explained for the propane molecule using very clear graphics. It is an excellent first exposure to the technique, and supplies a very solid…
This site offers an introductory course (as a MOOC) on the estimation of measurement uncertainty specifically related to chemical analysis (analytical chemistry). The course gives the main concepts and mathematical apparatus of measurement uncertainty estimation and introduces two principal approaches to measurement uncertainty estimation – the ISO GUM modeling approach (aa “bottom-up” approach) and the single-lab…
A set of slides that explain in detail the important aspects of interpreting EI mass spectra as obtained in GC-MS. The site explains how to use common losses (e.g. water), use of isotopic peaks, mass defects, the nitrogen rule and identifying the presence of rings and unsaturated bonds..
The website is available here .
A basic tutorial on the principles of Time-of-flight mass spectrometer design, presenting the concepts of ionization, acceleration from an ion source, energy dispersion of the ions due to space dispersion in the source, and the use of an ion mirror to focus this dispersion. The two-field source, the ion mirror, and the MCP ion detector is…
This site offers a concise, clearly-written introduction to general techniques of mass spectrometry. It includes descriptions and illustrations of various types of mass spectrometers and covers the major ionization techniques.The website is available here .
A set of slides that explain in detail the important aspects of interpreting EI mass spectra as obtained in GC-MS. The site explains how to use common losses (e.g. water), use of isotopic peaks, mass defects, the nitrogen rule and identifying the presence of rings and unsaturated bonds.The website is available here .
This publication highlights a collaboration between academia and industry to deliver a course of mass spectrometry. Highlighted in the text are instructor qualifications, instructor roles, student assignments, and student perceptions.The website is available here .
This chapter from David Harvey's complete e-text covers the general theory of column chromatography, GC, HPLC, and electrophoresis. Available as a downloadable pdf this 102 page chapter includes diagrams, examples and problems and provides an excellent introduction to separations.
The website is available here .
Horiba scientists wrote this tutorial to help people learn about Raman spectroscopy. The authors write clearly and provide great illustrations to accompany the text. The tutorial addresses the theory of Stokes & anti-Stokes Raman scattering, SERS and other techniques. In addition, the tutorial introduces the instruments needed for Raman experiments - like lasers, optics, and microscopes.…