A primer for liquid chromatography provided by Agilent Technologies. This publication reviews all aspects of fundamental liquid chromatography (LC). The material covered is similar to might be found in an analytical chemistry textbook. It is especially well-suited for first time users of liquid chromatography. Reviews of fundamental math, instrument design, and method development are included. In…
This chapter from David Harvey's complete e-text covers the general theory of column chromatography, GC, HPLC, and electrophoresis. Available as a downloadable pdf this 102 page chapter includes diagrams, examples and problems and provides an excellent introduction to separations.
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Designed as a faculty resource on separation science, with a particular emphasis on chromatographic separations. The published materials focus on active learning but may be modified to fit any curricular pedagogy. Materials include a text, sample problems, and an instructor's manual.
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Want to see what happens in LC, but there's not an HPLC available for your use? This simulator shows what the important parameters are and lets you see the effect of changing those parameters on realistic separations.
The website is available here .
Note - the software for this application is still functional, but runs on an outdated…
Microfluidics and electrophoresis are two aspects of bioanalysis that are central to modern instrumentation. This laboratory has students use a microfluidic device for electrophoretic experiments. It takes a microfabrication approach in contrast to, e.g., JASDL entry 10031.
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A complete textbook for Quantitative Analysis is freely downloadable. Teach yourself Analytical Chemistry, or be part of a class on the subject.
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Kinetic Capillary Electrophoresis is a family of methods for studying binding of biopolymers, measuring rate constants, binding constants, and in some cases providing mechanistic details. This annimation illustrates several kinetic CE modes and their effect on the complex formation and dissociation.
The website is available here .
This video, distributed on YouTube by the Royal Society of Chemistry is on the basic principles of GC. This video is a short primer (~5 min) which introduces the technique and instrumentation.
There are many assumptions that are made in the presentation of this material, due to the fact the video is meant to be a…
This video, distributed on YouTube by the Royal Society of Chemistry is on the basic principles of HPLC. This video is a short primer (~5 min) which introduces the basic technique and instrumentation. The theory of HPLC is described at a very basic level. Separation theory is presented in a qualitiative fashion, and both the mechanics…
This website contains an ion chromatography simulator that can be run on-line or can be downloaded. The simulator focuses on separations of proteins using Ion Chromatography. Also included is hyperlinked reference information, an example of a homework assignment using the simulator, and some information on protein structures.(note this is an archived version of this site, as…