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chromatography applications and methods

Analytical Chemistry 2.0: Chromatographic and Electrophoretic Methods

This chapter from David Harvey's complete e-text covers the general theory of column chromatography, GC, HPLC, and electrophoresis. Available as a downloadable pdf this 102 page chapter includes diagrams, examples and problems and provides an excellent introduction to separations. The website is available here .

Separation Science

Designed as a faculty resource on separation science, with a particular emphasis on chromatographic separations.  The published materials focus on active learning but may be modified to fit any curricular pedagogy. Materials include a text, sample problems, and an instructor's manual. The website is available here .

Gas Chromatography (EPA Field Methods)

US Environmental Protection Agency site that explains GC for those interested in environmental analysis. Assumes some user background in the field.  Includes a partial list of EPA Field Methods that utilize GC which may be helpful to those interested in regulatory protocols. The website is available here . Further Analytical Methods developed by the Environmental…

An Unusual Arson Case: Polymer Grains

A case example of determination of an arson accelerant by a variety of analytical techniques. Will make a good case record for an active learning class. Uses a variety of techniques, particularly chromatography and headspace analysis.The website is available here .

Affinity Chromatography

This is an experiment showing the application of affinity chromatography to the separation of albumin from horse serum. A brief introduction of affinity chromatography and how it is being used in this specific experiment is given. This appears to be a good experiment to show the advantages of affinity chromatography in separating specific proteins from a…
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