This chapter from David Harvey's complete e-text covers the general theory of column chromatography, GC, HPLC, and electrophoresis. Available as a downloadable pdf this 102 page chapter includes diagrams, examples and problems and provides an excellent introduction to separations.
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Designed as a faculty resource on separation science, with a particular emphasis on chromatographic separations. The published materials focus on active learning but may be modified to fit any curricular pedagogy. Materials include a text, sample problems, and an instructor's manual.
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A complete textbook for Quantitative Analysis is freely downloadable. Teach yourself Analytical Chemistry, or be part of a class on the subject.
The website is available here .
US Environmental Protection Agency site that explains GC for those interested in environmental analysis. Assumes some user background in the field. Includes a partial list of EPA Field Methods that utilize GC which may be helpful to those interested in regulatory protocols.
The website is available here .
Further Analytical Methods developed by the Environmental…
This site discusses many of the details in how ro convert a traditional HPLC system into a capillary HPLC system. Included in the discussion is how to use splitting to get to the lower flow rates required and how to pack your own capillary LC columns. There is detailed information on how to go about plumbing…
Applications and tutorials related to supercritical fluid chromatography and temperature programming in liquid chromatography (PDF). Application area is mainly petrochemicals.The website is available here .
This chromatography experiment is designed to introduce students to the theory of chromatographic separation of mixtures and packed column chromatography. A section on instrument troubleshooting may be included as an additional optional activity.
The website is available here .
A case example of determination of an arson accelerant by a variety of analytical techniques. Will make a good case record for an active learning class. Uses a variety of techniques, particularly chromatography and headspace analysis.The website is available here .
This is an experiment showing the application of affinity chromatography to the separation of albumin from horse serum. A brief introduction of affinity chromatography and how it is being used in this specific experiment is given. This appears to be a good experiment to show the advantages of affinity chromatography in separating specific proteins from a…
Analysis of arson with detailed information on quality control and methods of transfer, excellent sample chromatograms. Could be used as a problem based learning tool. Illustrates important aspects of headspace analysisThe website is available here .