A primer for liquid chromatography provided by Agilent Technologies. This publication reviews all aspects of fundamental liquid chromatography (LC). The material covered is similar to might be found in an analytical chemistry textbook. It is especially well-suited for first time users of liquid chromatography. Reviews of fundamental math, instrument design, and method development are included. In…
This chapter from David Harvey's complete e-text covers the general theory of column chromatography, GC, HPLC, and electrophoresis. Available as a downloadable pdf this 102 page chapter includes diagrams, examples and problems and provides an excellent introduction to separations.
The website is available here .
Designed as a faculty resource on separation science, with a particular emphasis on chromatographic separations. The published materials focus on active learning but may be modified to fit any curricular pedagogy. Materials include a text, sample problems, and an instructor's manual.
The website is available here .
Want to see what happens in LC, but there's not an HPLC available for your use? This simulator shows what the important parameters are and lets you see the effect of changing those parameters on realistic separations.
The website is available here .
Note - the software for this application is still functional, but runs on an outdated…
A complete textbook for Quantitative Analysis is freely downloadable. Teach yourself Analytical Chemistry, or be part of a class on the subject.
The website is available here .
This video, distributed on YouTube by the Royal Society of Chemistry is on the basic principles of HPLC. This video is a short primer (~5 min) which introduces the basic technique and instrumentation. The theory of HPLC is described at a very basic level. Separation theory is presented in a qualitiative fashion, and both the mechanics…
This website contains an ion chromatography simulator that can be run on-line or can be downloaded. The simulator focuses on separations of proteins using Ion Chromatography. Also included is hyperlinked reference information, an example of a homework assignment using the simulator, and some information on protein structures.(note this is an archived version of this site, as…
This is a link within the Waters HPLC Primer site. It contains very useful details regarding the properties of HPLC packing materials and how they impact performance. Since it is part of a larger site, it contains links to other information provided by waters on other aspects of HPLC as well as MS and other techniques.…
This site discusses many of the details in how ro convert a traditional HPLC system into a capillary HPLC system. Included in the discussion is how to use splitting to get to the lower flow rates required and how to pack your own capillary LC columns. There is detailed information on how to go about plumbing…
This animation site deals specifically with diode array detection for liquid chromatography. The animations are short (one to two minutes) and can easily be shown in class as part of a lecture. They are extremely helpful in illustrating key components and concepts of chromatographic systems. Users are encouraged to explore the site and the other brief…