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liquid chromatography

Primer for Liquid Chromatography – Agilent LC Handbook

A primer for liquid chromatography provided by Agilent Technologies. This publication reviews all aspects of fundamental liquid chromatography (LC).  The material covered is similar to might be found in an analytical chemistry textbook.  It is especially well-suited for first time users of liquid chromatography. Reviews of fundamental math, instrument design, and method development are included.  In…

Analytical Chemistry 2.0: Chromatographic and Electrophoretic Methods

This chapter from David Harvey's complete e-text covers the general theory of column chromatography, GC, HPLC, and electrophoresis. Available as a downloadable pdf this 102 page chapter includes diagrams, examples and problems and provides an excellent introduction to separations. The website is available here .

Separation Science

Designed as a faculty resource on separation science, with a particular emphasis on chromatographic separations.  The published materials focus on active learning but may be modified to fit any curricular pedagogy. Materials include a text, sample problems, and an instructor's manual. The website is available here .

HPLC – free, opensource HPLC simulator project

Want to see what happens in LC, but there's not an HPLC available for your use? This simulator shows what the important parameters are and lets you see the effect of changing those parameters on realistic separations. The website is available here . Note - the software for this application is still functional, but runs on an outdated…


This video, distributed on YouTube by the Royal Society of Chemistry is on the basic principles of HPLC. This video is a short primer (~5 min) which introduces the basic technique and instrumentation. The theory of HPLC is described at a very basic level. Separation theory is presented in a qualitiative fashion, and both the mechanics…

Ion Exchange Chromatography

This website contains an ion chromatography simulator that can be run on-line or can be downloaded. The simulator focuses on separations of proteins using Ion Chromatography. Also included is hyperlinked reference information, an example of a homework assignment using the simulator, and some information on protein structures.(note this is an archived version of this site, as…
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