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Tag: separations

Kinetic Capillary Electrophoresis(KCE): A conceptual Platform for Kinetic Homogeneous Affinity Methods

Kinetic Capillary Electrophoresis is a family of methods for studying binding of biopolymers, measuring rate constants, binding constants, and in some cases providing mechanistic details. This annimation illustrates several kinetic CE modes and their effect on the complex formation and dissociation. The website is available here .


This video, distributed on YouTube by the Royal Society of Chemistry is on the basic principles of HPLC. This video is a short primer (~5 min) which introduces the basic technique and instrumentation. The theory of HPLC is described at a very basic level. Separation theory is presented in a qualitiative fashion, and both the mechanics…

Ion Exchange Chromatography

This website contains an ion chromatography simulator that can be run on-line or can be downloaded. The simulator focuses on separations of proteins using Ion Chromatography. Also included is hyperlinked reference information, an example of a homework assignment using the simulator, and some information on protein structures.(note this is an archived version of this site, as…

Craig countercurrent extraction machine applet

This site provides a useful applet that demonstrates countercurrent extraction. The explanation of the method is short, but good. The level is appropriate for undergraduate students. The Applet itself is well designed and provides a useful exercise for students around which homework or small-group waork could be developed. The use of color is particularly helpful. The…

Applets for Chromatography, Signal Processing and General Analytical Chemistry

This site offers Java-based applets as organized in 4 categories: analytical and general chemistry, instrumental chemical analysis, instrumentation/signal processing, data analysis/chemometrics. Each applet includes a short introduction followed by a user controlled input of experimental conditions, such as seen for diffusion in electrochemistry.The website is available here .
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